As my Puffer Guru pointed out to me, I did miss a few. Basuto is a floating exhibit at the National Waterways Museum, Ellesmere Port, VIC 37 is/was at Maryport Maritime Museum and some of the very late motorships that operated into the 70's may still be knocking around in the world. VIC24 was laid up down Plymouth way but is probably gone now.
Kyles shifted to the Scottish Maritime Museum, Irvine base, after funding was withdrawn from Braehead base but I'm not sure she qualifies as a Puffer, although at 143 years old, she must be due some kind of award for longevity.
Thus, it can be said that the VIC32 is the last operational puffer in UK waters unless and until VIC27/Auld Reekie joins her.
The BBC 2 documentary gave the arrival of the CalMac ROROs as the reason for the demise of the puffers. What it didn't mention was that CalMac also received a Government subsidy that was never available to the puffers. Len Paterson's excellent 'Light in the Glens' gives a full explanation of this.
Barry M